Pullulanase
Pullulanase
is a specific kind of
glucanase
, an amylolytic
exoenzyme
, that degrades
pullulan
. It is produced as an extracellular, cell surface-anchored
lipoprotein
by
Gram-negative bacteria
of the genus
Klebsiella
. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are are also able to hydrolyse α-1,4 linkages. It is also produced by some other
bacteria
and
archaea
. Pullanase is used as a detergent in
biotechnology
.
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