Phage Display

1. The function of protein X is unknown. The protein is used to coat the surface of a small plastic dish.
2. All the other genes in the genome are expressed as fusions with the coat protein of a bacteriophage, so that they are displayed on the surface of the viral particle.
3. This phage-display library is added to the dish. After a while, the dish is washed.
4. Phage-displaying proteins that interact with protein X remain attached to the dish, while all others are washed away. DNA extracted from interacting phage contains the sequences of interacting proteins.

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