Peptide Sequence
Peptide sequence
or
amino acid sequence
is the order in which
amino acid
residues, connected by
peptide bonds
, lie in the chain. The sequence is generally reported from the N-terminal end containing free
amino group
to the C-terminal end containing free
carboxyl group
. Peptide sequence is often called
protein sequence
if it represent the
primary structure
of a
protein
. Several deductions can be made from the sequence. Long stretches of
hydrophobic
residues may indicate
transmembrane helices
. Certain residues indicate a
beta sheet
area. If full-lenght protein sequence is available, it is possible to estimate the
isoelectric point
of the protein. Methods for determining the peptide sequence include deduction from
DNA
sequence,
Edman degradation
, and
mass spectrometry
.
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