Numerical Aperture (Microscopy)
In
microscopy
, the
numerical aperture
,
A
N
, of an
objective
is:
A_N = I \sin \frac {a} {2}
where
I
is the
index of refraction
of the medium in which the lens is working (1.0 for
air
, up to 1.56 for
oils
), and
a
is the
angular aperture
of the
lens
. It is basically a measure of the diameter of the
aperture
compared to the
focal length
. In
photography
, the
f-number
expresses the same relationship.
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