Native Chemical Ligation
Native chemical ligation
is a technique for constructing a large
peptide
from two smaller peptides, a C-terminal
thioester
peptide and a N-terminal
cysteine
peptide. This process was developed by
Phillip Dawson
and
Steven Kent
at the
Scripps Research Institute
in 1987, about the same time that
inteins
were discoverd, which is nature's analogous reaction.
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