| Comments |
| Adai | Spoken in Texas and Louisiana, United States. |
| Aikan | Spoken in Rondnia, Brazil. |
| Alagilac | Spoken in Guatemala. |
| Andoque | Spoken in Colombia and Peru. |
| Atakapa | Spoken in Texas and Louisiana, United States. |
| Ainu | Endangered language that is spoken in northern Japan. |
| Baenan | Spoken in Brazil. |
| Basque | No known living relatives; found in the Basque region of France and Spain. Aquitanian is commonly regarded as a direct ancestor of Basque. Some linguists have claimed similarities with various languages of the Caucasus, especially because of its ergative case system, but the resemblances seem superficial. Other linguists have proposed a relation to Iberic. |
| Beothuk | Spoken in Newfoundland, Canada. |
| Betoi | Spoken in Colombia. |
| Birale (Ongota) | Often considered Afroasiatic. |
| Burushaski | Little information is available. |
| Calusa | Spoken in Florida, United States. |
| Cams | Spoken in Colombia. |
| Canichana | Spoken in Bolivia. |
| Cayubaba | Spoken in Bolivia. |
| Cayuse | Spoken in Oregon and Washington, United States. |
| Chimariko | Spoken in California, United States. |
| Chitimacha | Spoken in Louisiana, United States. |
| Coahuilteco | Spoken in Texas, United States and northeastern Mexico. |
| Cofn | Spoken in Colombia and Ecuador. |
| Cotoname | Spoken in Texas, United States and northeastern Mexico. |
| Cuitlatec | Spoken in Guerrero, Mexico. |
| Culle | Spoken in Peru. |
| Elamite | Extinct language of Elamite Empire. Some conjecture a relationship to the Dravidian languages. |
| Esselen | Spoken in California, United States. |
| Etruscan | Language of the ancient Etruscans in northwestern Italy; not well understood at present. |
| Gamela | Spoken in Maranho, Brazil. |
| Gorgotoqui | Spoken in Bolivia. |
| Hadza | Often listed as an outlier among the Khoisan languages. |
| Haida | Spoken in Alaska, United States and British Columbia, Canada. Some proposals to connect to Na-Den languages. |
| Huamo | Spoken in Pernambuco, Brazil. |
| Huave | Spoken in Oaxaca, Mexico. |
| Iberic | There are lexical coincidences with Basque, but it is hard to know if they are more than a result of vicinity. |
| Irantxe | Spoken in Mato Grosso, Brazil. |
| Itonama | Spoken in Bolivia. |
| Jalaa | An endangered language of northeastern Nigeria. |
| Japanese | Possibly related to Korean language, though not yet proven. Connections to the Altaic languages have also been proposed. See Altaic hypothesis for these theories. |
| Jot | Spoken in Venezuela. |
| Kalto | Also called Nahali, it is a near-extinct language of western India. |
| Karankawa | Spoken in Texas, United States. |
| Karir | Spoken in Paraba, Pernambuco, and Cear, Brazil. |
| Karok | Spoken in California, United States. |
| Ket | No known relatives. Some linguists have attempted to show a relationship with Burushaski. |
| Koay | Spoken in Rondnia, Brazil. |
| Konomihu | Spoken in California, United States. |
| Korean | Possibly related to Japanese language, though not yet proven. Connections to the Altaic languages have also been proposed. See the Altaic hypothesis for these theories. |
| Kukur | Spoken in Mato Grosso, Brazil. |
| Kutenai | Spoken in Idaho and Montana, United States and British Columbia, Canada. |
| Kwadi | Often listed as an outlier among the Khoisan languages. |
| Kusunda | Nearly extinct language of Nepal. Sometimes considered one of the Tibeto-Burman languages. |
| Laal | Unclassified; sometimes considered Niger-Congo. |
| Mapudungu | Spoken in Chile and Argentina. |
| Maratino | Spoken in northeastern Mexico. |
| Mekejir (Shabo) | Often considered Nilo-Saharan. |
| Meroitic | Extinct language of ancient kingdom of Meroe (Kush). |
| Movima | Spoken in Bolivia. |
| Munichi | Spoken in Peru. |
| Nambiquaran | Spoken in Mato Grosso, Brazil. |
| Naolan | Spoken in Tamaulipas, Mexico. |
| Natchez | Spoken in Mississippi and Louisiana, United States. |
| Nat | Spoken in Pernambuco, Brazil. |
| Nivkh | Also known as Gilyak. A Palaeosiberian language spoken in the lower Amur River basin and on the Sakhalin Islands. Ainu is also spoken on Sakhalin. |
| Omurano | Spoken in Peru. |
| Oropom | Possibly nonexistent. |
| Ot | Spoken in So Paulo, Brazil. |
| Pankarar | Spoken in Pernambuco, Brazil. |
| Pirah | Related to languages that have recently become extinct. Only known language with no number terminology, grammatical recursion, and several other unique features. |
| Puquina | Spoken in Bolivia. |
| Quinigua | Spoken in northeastern Mexico. |
| Sabela | Spoken in Ecuador and Peru. |
| Salinan | Spoken in California, United States. |
| Sandawe | Often listed as an outlier among the Khoisan languages. |
| Seri | Spoken in Sonora, Mexico. |
| Siuslaw | Spoken in Oregon, United States. |
| Solano | Spoken in Texas, United States and northeastern Mexico. |
| Sumerian | Long-extinct language of ancient Sumeria. |
| Taiap | |
| Takelma | Spoken in Oregon, United States. |
| Tarairi | Spoken in Rio Grande do Norte, Brazil. |
| Tarascan | Latest research suggests the possibility that it may be related to a family of languages from Ecuador. |
| Tasmanian | Some theories suggest that they split Australian Aboriginal languages 10,000 years ago, but no conclusive proof exists. |
| Taushiro | Spoken in Peru. |
| Tequiraca | Spoken in Peru. |
| Ticuna | Spoken in Colombia, Peru, and Brazil. |
| Timucua | Spoken in Florida and Georgia, United States. |
| Tonkawa | Spoken in Texas, United States. |
| Tunica | Spoken in Mississippi, Louisiana, and Arkansas, United States. |
| Tux | Spoken in Bahia and Pernambuco, Brazil. Now extinct. |
| Warao | Spoken in Guyana, Surinam, and Venezuela. |
| Washo | Spoken in California and Nevada, United States. |
| Xok | Spoken in Alagoas and Pernambuco, Brazil. |
| Xukur | Spoken in Pernambuco and Paraba, Brazil. |
| Ymana | Spoken in Chile. |
| Yana | Spoken in California, United States. |
| Yuchi | Spoken in Georgia and Oklahoma, United States. Connections to Siouan languages have been proposed. |
| Yukaghir | Connections to Uralic Languages have been proposed. |
| Yuracare | Spoken in Bolivia. |
| Yuri | Spoken in Colombia and Brazil. |
| Yurumangu | Spoken in Colombia. |
| Zuni | Spoken in New Mexico, United States. Connections to Penutian languages have been proposed. |
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