Hepes
Hepes
(
4-2-hydroxyethyl-1-piperazineethanesulfonic acid
) is an organic chemical
buffer
which is widely used in cell culture to maintain physiological pH. Its
molecular weight
is 238.3. Hepes is recommended for the protection of frozen solutions of enzymes from freezing-induced pH. Fears that Hepes may serve as a nutrient source for
aerobic bacteria
have been shown to be unfounded.
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