Koch's Postulates

1. The organism must be found in all animals suffering from the disease, but not in healthy animals.
2. The organism must be isolated from a diseased animal and grown in pure culture.
3. The cultured organism should cause disease when introduced into a healthy animal.
4. The organism must be reisolated from the experimentally infected animal.

Reference

• Koch R. ber die tiologie der Tuberkulose. In: "Verhandlungen des Kongresses fr Innere Medizin. Erster Kongress, Wiesbaden 1882".

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